AS/NZS 4276.6:2007

1-AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
1-PREFACE
2-METHOD
2-1 SCOPE
2-2 REFERENCED DOCUMENTS
2-3 DEFINITIONS
2-3.1 Coliforms
2-3.2 Thermotolerant coliforms
2-3.3 Escherichia coli (E. coli)
2-4 PRINCIPLE
3-5 CULTURE MEDIA AND REAGENTS (See Appendix B)
3-6 APPARATUS
3-7 REFERENCE CULTURES
3-7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
3-7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
3-7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
3-7.4 Use of reference cultures
4-8 SAMPLES
4-8.1 Laboratory samples
4-8.2 Dilutions
4-9 PROCEDURE
4-9.1 Presumptive test
5-9.2 Confirmatory tests
5-9.2.1 Coliforms
5-9.2.2 E. coli
6-9.2.3 Thermotolerant coliforms
6-10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
6-11 TEST REPORT
6-12 BIBLIOGRAPHY
7-APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS-MOST PROBABLE NUMBER
8-APPENDIX B - CULTURE MEDIA AND REAGENTS
8-B1 SCOPE
8-B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
10-B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
10-B4 NUTRIENT AGAR
11-B5 MACCONKEY AGAR
11-B6 OXIDASE REAGENT (KOVACS)
11-B7 EC-MUG BROTH
12-APPENDIX C - PERFORMANCE OF KOVACS OXIDASE TEST